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stem pro adipogenesis differentiation medium  (Thermo Fisher)


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    Structured Review

    Thermo Fisher stem pro adipogenesis differentiation medium
    Adipocyte differentiation of KNP-MSCs and HNT-MSCs. ( A ) KNP-MSCs and HNT-MSCs were cultured with a standardized <t>adipogenesis</t> differentiation medium and, after 20 days, stained with Oil Red O to detect lipid droplet amount under 40× magnification of phase contrast microscopy. Scale bar: 100 μm. ( B , C ) ImageJ Fiji software analyses were performed to quantify the percentage of red stained area and the mean of red intensity after 20 days of adipogenic differentiation. ( D , E ) Triglyceride-Glo™Assay was performed to assess triacylglycerols and glycerol levels after 20 days of adipogenic differentiation. ( F – I ) The adipogenic differentiation-related gene expression of PPARγ2, FABP4, Adipo-Q, and LPL were evaluated by RT-qPCR after 10 and 20 days in adipogenic differentiation medium, using the housekeeping gene GAPDH for normalization. Data are displayed as means ± SD from three different experiments (** p < 0.001; *** p < 0.0001).
    Stem Pro Adipogenesis Differentiation Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stem pro adipogenesis differentiation medium/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    stem pro adipogenesis differentiation medium - by Bioz Stars, 2026-03
    86/100 stars

    Images

    1) Product Images from "Cellular and Biochemical Characterization of Mesenchymal Stem Cells from Killian Nasal Polyp"

    Article Title: Cellular and Biochemical Characterization of Mesenchymal Stem Cells from Killian Nasal Polyp

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms232113214

    Adipocyte differentiation of KNP-MSCs and HNT-MSCs. ( A ) KNP-MSCs and HNT-MSCs were cultured with a standardized adipogenesis differentiation medium and, after 20 days, stained with Oil Red O to detect lipid droplet amount under 40× magnification of phase contrast microscopy. Scale bar: 100 μm. ( B , C ) ImageJ Fiji software analyses were performed to quantify the percentage of red stained area and the mean of red intensity after 20 days of adipogenic differentiation. ( D , E ) Triglyceride-Glo™Assay was performed to assess triacylglycerols and glycerol levels after 20 days of adipogenic differentiation. ( F – I ) The adipogenic differentiation-related gene expression of PPARγ2, FABP4, Adipo-Q, and LPL were evaluated by RT-qPCR after 10 and 20 days in adipogenic differentiation medium, using the housekeeping gene GAPDH for normalization. Data are displayed as means ± SD from three different experiments (** p < 0.001; *** p < 0.0001).
    Figure Legend Snippet: Adipocyte differentiation of KNP-MSCs and HNT-MSCs. ( A ) KNP-MSCs and HNT-MSCs were cultured with a standardized adipogenesis differentiation medium and, after 20 days, stained with Oil Red O to detect lipid droplet amount under 40× magnification of phase contrast microscopy. Scale bar: 100 μm. ( B , C ) ImageJ Fiji software analyses were performed to quantify the percentage of red stained area and the mean of red intensity after 20 days of adipogenic differentiation. ( D , E ) Triglyceride-Glo™Assay was performed to assess triacylglycerols and glycerol levels after 20 days of adipogenic differentiation. ( F – I ) The adipogenic differentiation-related gene expression of PPARγ2, FABP4, Adipo-Q, and LPL were evaluated by RT-qPCR after 10 and 20 days in adipogenic differentiation medium, using the housekeeping gene GAPDH for normalization. Data are displayed as means ± SD from three different experiments (** p < 0.001; *** p < 0.0001).

    Techniques Used: Cell Culture, Staining, Microscopy, Software, Glo Assay, Expressing, Quantitative RT-PCR



    Similar Products

    stem pro adipogenesis differentiation medium  (Thermo Fisher)
    86
    Thermo Fisher stem pro adipogenesis differentiation medium
    Adipocyte differentiation of KNP-MSCs and HNT-MSCs. ( A ) KNP-MSCs and HNT-MSCs were cultured with a standardized <t>adipogenesis</t> differentiation medium and, after 20 days, stained with Oil Red O to detect lipid droplet amount under 40× magnification of phase contrast microscopy. Scale bar: 100 μm. ( B , C ) ImageJ Fiji software analyses were performed to quantify the percentage of red stained area and the mean of red intensity after 20 days of adipogenic differentiation. ( D , E ) Triglyceride-Glo™Assay was performed to assess triacylglycerols and glycerol levels after 20 days of adipogenic differentiation. ( F – I ) The adipogenic differentiation-related gene expression of PPARγ2, FABP4, Adipo-Q, and LPL were evaluated by RT-qPCR after 10 and 20 days in adipogenic differentiation medium, using the housekeeping gene GAPDH for normalization. Data are displayed as means ± SD from three different experiments (** p < 0.001; *** p < 0.0001).
    Stem Pro Adipogenesis Differentiation Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stem pro adipogenesis differentiation medium/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    stem pro adipogenesis differentiation medium - by Bioz Stars, 2026-03
    86/100 stars
    		  Buy from Supplier
    stem pro® adipogenesis differentiation medium  (Thermo Fisher)
    90
    Thermo Fisher stem pro® adipogenesis differentiation medium
    Adipocyte differentiation of KNP-MSCs and HNT-MSCs. ( A ) KNP-MSCs and HNT-MSCs were cultured with a standardized <t>adipogenesis</t> differentiation medium and, after 20 days, stained with Oil Red O to detect lipid droplet amount under 40× magnification of phase contrast microscopy. Scale bar: 100 μm. ( B , C ) ImageJ Fiji software analyses were performed to quantify the percentage of red stained area and the mean of red intensity after 20 days of adipogenic differentiation. ( D , E ) Triglyceride-Glo™Assay was performed to assess triacylglycerols and glycerol levels after 20 days of adipogenic differentiation. ( F – I ) The adipogenic differentiation-related gene expression of PPARγ2, FABP4, Adipo-Q, and LPL were evaluated by RT-qPCR after 10 and 20 days in adipogenic differentiation medium, using the housekeeping gene GAPDH for normalization. Data are displayed as means ± SD from three different experiments (** p < 0.001; *** p < 0.0001).
    Stem Pro® Adipogenesis Differentiation Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stem pro® adipogenesis differentiation medium/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    stem pro® adipogenesis differentiation medium - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Adipocyte differentiation of KNP-MSCs and HNT-MSCs. ( A ) KNP-MSCs and HNT-MSCs were cultured with a standardized adipogenesis differentiation medium and, after 20 days, stained with Oil Red O to detect lipid droplet amount under 40× magnification of phase contrast microscopy. Scale bar: 100 μm. ( B , C ) ImageJ Fiji software analyses were performed to quantify the percentage of red stained area and the mean of red intensity after 20 days of adipogenic differentiation. ( D , E ) Triglyceride-Glo™Assay was performed to assess triacylglycerols and glycerol levels after 20 days of adipogenic differentiation. ( F – I ) The adipogenic differentiation-related gene expression of PPARγ2, FABP4, Adipo-Q, and LPL were evaluated by RT-qPCR after 10 and 20 days in adipogenic differentiation medium, using the housekeeping gene GAPDH for normalization. Data are displayed as means ± SD from three different experiments (** p < 0.001; *** p < 0.0001).

    Journal: International Journal of Molecular Sciences

    Article Title: Cellular and Biochemical Characterization of Mesenchymal Stem Cells from Killian Nasal Polyp

    doi: 10.3390/ijms232113214

    Figure Lengend Snippet: Adipocyte differentiation of KNP-MSCs and HNT-MSCs. ( A ) KNP-MSCs and HNT-MSCs were cultured with a standardized adipogenesis differentiation medium and, after 20 days, stained with Oil Red O to detect lipid droplet amount under 40× magnification of phase contrast microscopy. Scale bar: 100 μm. ( B , C ) ImageJ Fiji software analyses were performed to quantify the percentage of red stained area and the mean of red intensity after 20 days of adipogenic differentiation. ( D , E ) Triglyceride-Glo™Assay was performed to assess triacylglycerols and glycerol levels after 20 days of adipogenic differentiation. ( F – I ) The adipogenic differentiation-related gene expression of PPARγ2, FABP4, Adipo-Q, and LPL were evaluated by RT-qPCR after 10 and 20 days in adipogenic differentiation medium, using the housekeeping gene GAPDH for normalization. Data are displayed as means ± SD from three different experiments (** p < 0.001; *** p < 0.0001).

    Article Snippet: To induce adipogenic differentiation, 1 × 10 4 cells/cm2 were seeded in 12-well plates and incubated for 20 days with STEM PRO ® Adipogenesis differentiation medium (cat. No. A1007001 Life Technologies).

    Techniques: Cell Culture, Staining, Microscopy, Software, Glo Assay, Expressing, Quantitative RT-PCR